Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Toll Like Receptor 4 (TLR4)
CD284; TOLL; HToll
- 編號MEA753Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.122ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL，37°C溫育1小時；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實驗原理
將Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA753Hu01 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA753Hu01 | Toll樣受體4(TLR4)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA753Hu02 | Toll樣受體4(TLR4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA753Hu02 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Hu03 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Hu04 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA753Hu21 | Toll樣受體4(TLR4)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA753Hu02 | Toll樣受體4(TLR4)多克隆抗體 | IHC |
| PAA753Hu01 | Toll樣受體4(TLR4)多克隆抗體 | WB; FCM |
| PAA753Hu08 | Toll樣受體4(TLR4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA753Hu71 | Toll樣受體4(TLR4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA753Hu25 | Toll樣受體4(TLR4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA753Hu24 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| MAA753Hu23 | Toll樣受體4(TLR4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA753Hu21 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| MAA753Hu22 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| LAA753Hu72 | Toll樣受體4(TLR4)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MEA753Hu | Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA753Hu | Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA753Hu | Toll樣受體4(TLR4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA753Hu01 | Toll樣受體4(TLR4)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA753Hu01 | Toll樣受體4(TLR4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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