Toll樣受體4(TLR4)多克隆抗體
Polyclonal Antibody to Toll Like Receptor 4 (TLR4)
CD284; TOLL; HToll
- 編號PAA753Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPA753Hu01-Toll樣受體4(TLR4)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.21mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 569¥ 1327¥ 1896¥ 4740¥ 18960
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Liver lysate; Lane3: Rat Testis lysate Primary Ab: 1μg/ml Rabbit Anti-Human TLR4 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Human SH-SY5Y cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human TLR4 Polyclonal Antibody (Catalog PAA753Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對TLR4的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別TLR4。
用法
Western blotting: 0.01-2μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA753Hu01 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA753Hu01 | Toll樣受體4(TLR4)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA753Hu02 | Toll樣受體4(TLR4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA753Hu02 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Hu03 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA753Hu04 | Toll樣受體4(TLR4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA753Hu21 | Toll樣受體4(TLR4)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA753Hu02 | Toll樣受體4(TLR4)多克隆抗體 | IHC |
| PAA753Hu01 | Toll樣受體4(TLR4)多克隆抗體 | WB; FCM |
| PAA753Hu08 | Toll樣受體4(TLR4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA753Hu71 | Toll樣受體4(TLR4)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA753Hu25 | Toll樣受體4(TLR4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA753Hu24 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| MAA753Hu23 | Toll樣受體4(TLR4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA753Hu21 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| MAA753Hu22 | Toll樣受體4(TLR4)單克隆抗體 | WB |
| LAA753Hu72 | Toll樣受體4(TLR4)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| MEA753Hu | Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA753Hu | Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA753Hu | Toll樣受體4(TLR4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA753Hu01 | Toll樣受體4(TLR4)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA753Hu01 | Toll樣受體4(TLR4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
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| Journal For?Neurovirology | Soluble?TLR2?and?4?concentrations?in?cerebrospinal?fluid?in?HIV/SIV-relatedneuropathological?conditions.[pubmed:27882497] |
| Acta?Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints[pubmed:28093922] |
| Environmental Toxicology and Pharmacology | Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study[pubmed:28192751] |
| Inflammation | Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats[pubmed:28690610] |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[DOI: 10.1016/j.cimid.2017.06.002] |
| Comparative Immunology, Microbiology and Infectious Diseases | Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts[10.1016/j.cimid.2017.06.002] |
| Microbial Pathogenesis | Protective effects of sinomenine against LPS-induced inflammation in piglets.[pubmed:28757275] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects[pubmed:29102164] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms[pubmed:28061403] |
| Frontiers in Immunology | The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker[Pubmed:29593720] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia[Pubmed:30071285] |
| Neurotoxicity Research | Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells[Pubmed:29869772] |
| European?Journal of Pharmacology | Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1[Pubmed:29920283] |
| Environmental Toxicology and Pharmacology | Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity[Pubmed:29625388] |
| Physiology &?Behavior? | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll?…[Pubmed:29410018] |
| International Journal of Gynecology & Obstetrics | Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia[Pubmed:29569400] |
| Journal of Maternal-Fetal?&?Neonatal?Medicine | Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state[Pubmed:29534643] |
| ТLR4-Р?ВН? У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВН?СТЮ ЗАХВОРЮВАННЯ[:] | |
| Journal of?Medical?Virology | Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in mice[Pubmed: 30281823] |
| Metabolic?Brain?Disease | Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms[Pubmed: 30456649] |
| Experimental?and?Therapeutic?Medicine | The HMGB1?RAGE/TLR?TNF?α signaling pathway may contribute to kidney injury induced by hypoxia[] |
| Immunopharmacology?and?immunotoxicology | Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis[Pubmed: 30488751] |
| International Immunopharmacology | S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation[Pubmed: 30665040] |
| Biomedicine?& Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4[] |
| International Journal of Obesity | Circulating microbiota-derived metabolites: a “l(fā)iquid biopsy?[Pubmed: 31388096] |
| Scientific Reports | Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome[Pubmed: 31363123] |
| Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis[Pubmed: 31465731] |
| Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis[Pubmed: 31600583] |
| Thrombosis and haemostasis | Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in Children[Pubmed: 31858520] |
| J Ethnopharmacol | Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea[Pubmed: 33217518] |
| International Journal of Molecular Sciences | Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis[Pubmed: 32545403] |
| NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms[Pubmed: 32526752] |
| journal of biochemical and molecular toxicology | S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d‐galactosamine in mouse: Underlying mechanisms[Pubmed: 32453893] |
| microbial pathogenesis | Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice[Pubmed: 32389705] |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …[] |
| Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial?…[] | |
| The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children[] | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces[34091792] |
| Eksp Klin Gastroenterol | Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis[17539352] |
| Iranian Journal of Medical Sciences | Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice[] |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis[34244993] |
| Nutrients | Expression of Jejunal Taste Receptors in Women with Morbid Obesity[34371946] |
| Biomed Pharmacother | Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and?…[Pubmed:35220029] |
| Biomed Pharmacother | Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an?…[Pubmed:35202914] |
| Frontiers in Pharmacology | Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Front?…[Pubmed:35754464] |
| Processes | Lipocalin, Resistin and Gut Microbiota-Derived Propionate Could Be Used to Predict Metabolic Bariatric Surgery Selected Outcomes[] |
| Biomolecules | Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study[] |