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攝食抑制因子1(NES1)多克隆抗體

Polyclonal Antibody to Nesfatin 1 (NES1)

編號(hào)PAA242Mu01
物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
來源多抗制備
宿主兔
效價(jià)-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原RPA242Mu01-攝食抑制因子1(NES1)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度500μg/mL
且適物種-
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse NES1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse NES1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse NES1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse NES1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant NES1, Mouse.
Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Heart lysate;
Primary Ab: 1μg/mL Rabbit Anti-Mouse NES1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)NES1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別NES1。

用法

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
TBST 緩沖液
 TBS緩沖液
 TRIS緩沖液

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Bulletin of Clinical Psychopharmacology	Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder[Psikofarmakoloji: 2043]
Progress in Neuro-Psychopharmacology & Biological Psychiatry	High plasma nesfatin-1 level in patients with major depressive disorder[PubMed: 21163317]
Endocrine	Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus[SpringerLink: h978088657555w16]
Bulletin of Clinical Psychopharmacology	Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212]
Psychoneuroendocrinology.	Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder[PubMed: 22595767]
Iranian Journal of Health and Physical activity	Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats[Ijvst: Source]
European Journal of Obstetrics & Gynecology and Reproductive Biology	Decreased serum nesfatin-1 levels in endometriosis[Pubmed: 24702902]
Bulletin of Clinical Psychopharmacology	Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.[Psikofarmakoloji: Source]
Current Pharmaceutical Design	Role of NUCB2/nesfatin-1 as a possible biomarker.[Pubmed: 23537082]
Regulatory, Integrative and Comparative Physiology	A role of nesfatin-1/NucB2 in dehydration-induced anorexia[Pubmed:24829503]
J Endocrinol Invest.	Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome[Pubmed:24920281]
Int J Surg.	The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.[Pubmed:25448651]
Hormone molecular biology and clinical investigation	The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers[Pubmed:25581765]
Turk J Gastroenterol.	New hormones to predict the severity of gallstone-induced acute pancreatitis[Pubmed:25599787]
Respiration	Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?[PubMed: 26088934]
J Clin Res Pediatr Endocninol	Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children[PubMed: 26831553]
Bosn J Basic Med Sci	Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome[PubMed: 26295295]
Original Article / Orijinal Makale	Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1[Sayilar: 294]
Gynecol Endocrinol	Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome[PubMed: 26062107]
International Journal of Neuroscience	Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*[Pubmed:26872406]
未找到	Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment[P.1.e.012.pdf]
International Journal of Developmental	Serum?nesfatin-1?levels: a?potential?new?biomarker?in?patients?with?subarachnoid?hemorrhage.[pubmed:26872406]
Current Pharmaceutical Design	Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease[pubmed:28137218]
Arch Endocrinol Metab	Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes[pubmed:28977160]
Indian Journal of Psychiatry	Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?[pubmed:28529367]
Biomedicine & Pharmacotherapy	Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ[Pubmed:29604590]
Biochimica et?Biophysica?Acta	Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling[Pubmed:29627363]
BMC Psychiatry	Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients[Pubmed:29615007]
Probiotics?and Antimicrobial?Proteins	Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar[Pubmed: 30232744]
	An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages[]
clinical neurology and neurosurgery	Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels[Pubmed: 32311618]
Ann Nutr Metab	Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity[Pubmed: 33027789]
Endokrynologia Polska	Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke[33283259]
	Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder[]

編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA242Mu01	攝食抑制因子1(NES1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA242Mu01	攝食抑制因子1(NES1)活性蛋白	Cell?culture;?Activity?Assays.
PAA242Mu01	攝食抑制因子1(NES1)多克隆抗體	WB; IHC; ICC; IP.
SEA242Mu	攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
CEA242Mu	攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
MEA242Mu	攝食抑制因子1(NES1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA242Mu	攝食抑制因子1(NES1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

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