白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)
Mini Samples ELISA Kit for Interleukin 2 (IL2)
TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
- 編號(hào)MEA073Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍15.6-1,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于5.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-94 | 89 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 80-102 | 79 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 93-105% | 79-94% | 82-103% |
| EDTA plasma(n=5) | 95-103% | 81-92% | 98-105% | 85-99% |
| heparin plasma(n=5) | 78-94% | 90-101% | 80-89% | 88-97% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL，37°C溫育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A25µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA073Hu01 | 白介素2(IL2)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA073Hu02 | 白介素2(IL2)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPA073Hu61 | 白介素2(IL2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA073Hu01 | 白介素2(IL2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA073Hu61 | 白介素2(IL2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA073Hu02 | 白介素2(IL2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA073Hu01 | 白介素2(IL2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA073Hu06 | 白介素2(IL2)多克隆抗體 | WB |
| FAA073Hu01 | 抗白介素2(IL2)多克隆抗體 | Flow cytometry. |
| LAA073Hu71 | 白介素2(IL2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA073Hu26 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA073Hu21 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA073Hu22 | 白介素2(IL2)單克隆抗體 | WB |
| MAA073Hu24 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA073Hu25 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA073Hu23 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA073Hu27 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA073Hu29 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| HEA073Hu | 白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA073Hu | 白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA073Hu | 白介素2(IL2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA073Hu | 白介素2(IL2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA073Hu | 白介素2(IL2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA073Hu01 | 白介素2(IL2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice[34960627] |
| Animal Nutrition | Optimizing the growth and immune system of dairy calves by subdividing the pre-weaning period and providing different milk volumes for each stage[34786502] |
| Cancer Commun (Lond) | Anti‐PD‐L1/TGF‐βR fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer[Pubmed:34981670] |
| Int J Mol Sci | NLRP3 Inflammasome Activation Controls Vascular Smooth Muscle Cells Phenotypic Switch in Atherosclerosis[Pubmed:35008765] |
| Scientific Reports | A randomized controlled trial evaluating the effect of two low-level laser irradiation protocols on the rate of canine retraction[Pubmed:35710926] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis[Pubmed:35126715] |
| Toxicology Research | Alleviation of chemotherapy-induced acute lung injury via NLRP3/ASC/Caspase-1 signaling pathway[Pubmed:35782645] |
| Vaccines | Age-Dependent Dynamics of Maternally Derived Antibodies (MDAs) and Understanding MDA-Mediated Immune Tolerance in Foot-and-Mouth Disease-Vaccinated?…[Pubmed:35632433] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows[] |
