血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
- 編號SEA143Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.2pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價(jià)格¥ 2176¥ 3108¥ 13986¥ 26418¥ 217560
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測血管內(nèi)皮生長因子A(VEGFA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血管內(nèi)皮生長因子A(VEGFA)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-102 | 98 |
| EDTA plasma(n=5) | 83-91 | 88 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管內(nèi)皮生長因子A(VEGFA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管內(nèi)皮生長因子A(VEGFA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 79-90% | 81-98% | 99-105% |
| EDTA plasma(n=5) | 80-95% | 91-98% | 93-101% | 84-99% |
| heparin plasma(n=5) | 97-104% | 96-105% | 95-102% | 80-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將血管內(nèi)皮生長因子A(VEGFA)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的血管內(nèi)皮生長因子A(VEGFA)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管內(nèi)皮生長因子A(VEGFA)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管內(nèi)皮生長因子A(VEGFA)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA143Hu02 | 血管內(nèi)皮生長因子A(VEGFA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA143Hu01 | 血管內(nèi)皮生長因子A(VEGFA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA143Hu61 | 血管內(nèi)皮生長因子A(VEGFA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA143Hu01 | 血管內(nèi)皮生長因子A(VEGFA)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA143Hu01 | 血管內(nèi)皮生長因子A(VEGFA)多克隆抗體 | WB; IHC |
| PAA143Hu02 | 血管內(nèi)皮生長因子A(VEGFA)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA143Hu71 | 血管內(nèi)皮生長因子A(VEGFA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA143Hu81 | 血管內(nèi)皮生長因子A(VEGFA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| RAA143Hu21 | 血管內(nèi)皮生長因子A(VEGFA)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA143Hu24 | 血管內(nèi)皮生長因子A(VEGFA)單克隆抗體 | WB |
| MAA143Hu26 | 血管內(nèi)皮生長因子A(VEGFA)單克隆抗體 | WB |
| MAA143Hu21 | 血管內(nèi)皮生長因子A(VEGFA)單克隆抗體 | WB |
| FAA143Hu02 | 抗血管內(nèi)皮生長因子A(VEGFA)單克隆抗體 | Flow cytometry. |
| LAA143Hu76 | 血管內(nèi)皮生長因子A(VEGFA)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LMA143Hu | 血管內(nèi)皮生長因子A(VEGFA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| HEA143Hu | 血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA143Hu | 血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA143Hu | 血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA143Hu | 血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| PSA143Hu01 | 血管內(nèi)皮生長因子A(VEGFA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA143Hu01 | 血管內(nèi)皮生長因子A(VEGFA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| ACS Applied Materials & Interfaces | Tazarotene Released from Aligned Electrospun Membrane Facilitates Cutaneous Wound Healing by Promoting Angiogenesis[Pubmed: 31503444] |
| Journal of Ethnopharmacology | BuZangTongLuo decoction improved hindlimb ischemia by activating angiogenesis and regulating gut microbiota in diabetic mice[Pubmed: 31654796] |
| BIOFACTORS | Ginkgolide B and bilobalide promote the growth and increase β‐catenin expression in hair follicle dermal papilla cells of American minks[Pubmed: 31520488] |
| Acta Biomaterialia | Silicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheep[Pubmed: 31678741] |
| Folia Morphologica | Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression[Pubmed: 31802473] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea[Pubmed: 31882827] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | H19 regulates angiogenic capacity of extravillous trophoblasts by H19/miR-106a-5p/VEGFA axis[Pubmed: 32107606] |
| BMC Cancer | Syntenin overexpression in human lung cancer tissue and serum is associated with poor prognosis[Pubmed: 32106836] |
| Biomed Pharmacother | Soufeng Yuchuan decoction mitigates the ovalbumin-induced lung damage in a rat model of asthma[Pubmed: 32036215] |
| EBioMedicine | Canagliflozin impairs blood reperfusion of ischaemic lower limb partially by inhibiting the retention and paracrine function of bone marrow derived …[Pubmed: 31981975] |
| Clinical Hemorheology and Microcirculation | Nonselective alpha-/beta-AR antagonists can inhibit pericyte proliferation, migration, and secretion in vitro[Pubmed: 32224529] |
| Molecular Medicine Reports | Long non?coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR?590?3p under hypoxic conditions[Pubmed: 32323787] |
| Chinese medicine | The curative effects of the traditional Chinese herbal medicine “Jinchuang ointment” on excisional wounds[Pubmed: 32377230] |
| FASEB JOURNAL | Cyclin G2 upregulation impairs migration, invasion, and network formation through RNF123/Dvl2/JNK signaling in the trophoblast cell line HTR8/SVneo, a possible?…[Pubmed: 33205477] |
| Acta Histochem | Paeoniflorin accelerates foot wound healing in diabetic rats though activating the Nrf2 pathway[Pubmed: 33166863] |
| Reproductive Sciences | Maternal Serum VEGF Predicts Abnormally Invasive Placenta Better than NT-proBNP: a Multicenter Case-Control Study[Pubmed: 33025531] |
| Life Sci | Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy[Pubmed: 32497630] |
| ACS NANO | Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling[Pubmed: 32413258] |
| Neuropsychiatric Disease and Treatment | Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation[Pubmed: 32368063] |
| Scientific Reports | Molecular mechanisms of synergistic action of Ramucirumab and Paclitaxel in Gastric Cancers cell lines[Pubmed: 32346056] |
| carbohydrate polymers | Effect of Chitosan oligosaccharides on ischemic symptom and gut microbiota disbalance in mice with hindlimb ischemia[Pubmed: 32475560] |
| Cutaneous and Ocular Toxicology | Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?[Pubmed: 32722957] |
| Menoufia Medical Journal | Study of serum level of vascular endothelial growth factor in patients with hepatocellular carcinoma[] |
| Acta Scientific Women's Health | Modulatory Effects of Taurine Combined with Letrozole on Ehrlich Ascites Carcinoma in Mice[] |
| Indian Journal of Public Health Research & Development | Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever[] |
| Materials Science and Engineering: C | Cell-modified bioprinted microspheres for vascular regeneration[] |
| Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats[] | |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care[33469783] |
| Advanced Healthcare Materials | Accelerated Bone Regeneration by MOF Modified Multifunctional Membranes through Enhancement of Osteogenic and Angiogenic Performance[33448103] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water[33428732] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | In-vivo evaluation of alginate-pectin hydrogel film loaded with simvastatin for diabetic wound healing in Streptozotocin-induced diabetic rats[33421467] |
| Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ?-[33705802] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia[33878473] |
| Journal of Tissue Engineering | Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders[33868627] |
| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1|á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model[33789142] |
| Randomized Controlled Trial | Effect of Freeze-Dried Amnion Graft on Cytokines in Uterine Exudates Following Hysteroscopic Adhesiolysis of Severe Intrauterine Adhesions[28170094] |
| cancer communications | Programmed death ligand?\1 regulates angiogenesis and metastasis by participating in the c?\JUN/VEGFR2 signaling axis in ovarian cancer[33939321] |
| ?D1ú??éú3??§ó???éú3?2??ó?? | VEGFA/VEGFR2 ?úD?êó???à·????òòê×é?ˉ?a1üéú3é?Dμ?±í′??°×÷ó?[] |
| Chinese Journal of Parasitology and parasitic diseases | Expression and angiogenic effect of VEGFA/VEGFR2 in mice hepatic metacestode tissue of Echinococcus multilocularis[] |
| International Journal of Life science and Pharma Research | Artificial Sweeteners Alter Early Vitelline Vessel Development in Chick Embryo.(2021)[] |
| Biomed Mater | Preclinical efficacy study of a porous biopolymeric scaffold based on (gelatin-hyaluronic acid-chondroitin sulfate) in a porcine burn injury model: Role of critical?…[34384056] |
| J Cell Mol Med | Mesenchymal stem cells from different sources show distinct therapeutic effects in hyperoxia‐induced bronchopulmonary dysplasia in rats[34322990] |
| J Biochem Mol Toxicol | Thymoquinone upregulates miR‐125a‐5p, attenuates STAT3 activation, and potentiates doxorubicin antitumor activity in murine solid Ehrlich carcinoma[34605108] |
| Bioengineered | MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2: Running title: miR934?…[34699325] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum[34472855] |
| Cells | A-Kinase Anchoring Protein 2 Promotes Protection against Myocardial Infarction[34831084] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats[Pubmed:34986543] |
| Stem Cell Res Ther | A dual role of HIF1α in regulating osteogenesis–angiogenesis coupling[Pubmed:35123567] |
| American Journal of Translational Research | Curcumin treats endometriosis in mice by the HIF signaling pathway[Pubmed:35559378] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients[Pubmed:35348620] |
| Medicina | Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A?…[Pubmed:35208526] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?…[Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats[] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats[] |
| Research Square | DMOG attenuates steroid-associated endothelial progenitor cell impairment and osteonecrosis of the femoral head via regulating HIF-1α signaling pathway in?…[] |
