瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
- 編號SEA084Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍0.156-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.058ng/mL.
- 樣本類型serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2092¥ 2988¥ 13446¥ 25398¥ 209160
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實(shí)驗(yàn)結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測瘦素(LEP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的瘦素(LEP)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 78-90 | 82 |
| heparin plasma(n=5) | 88-104 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的瘦素(LEP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中瘦素(LEP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 86-101% | 99-105% | 86-94% |
| EDTA plasma(n=5) | 80-101% | 91-101% | 90-99% | 91-101% |
| heparin plasma(n=5) | 79-101% | 85-102% | 79-101% | 96-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將瘦素(LEP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的瘦素(LEP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的瘦素(LEP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的瘦素(LEP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA084Hu01 | 瘦素(LEP)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA084Hu01 | 瘦素(LEP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA084Hu01 | 瘦素(LEP)多克隆抗體 | WB; IHC |
| LAA084Hu71 | 瘦素(LEP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA084Hu81 | 瘦素(LEP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA084Hu22 | 瘦素(LEP)單克隆抗體 | WB; IHC |
| MAA084Hu21 | 瘦素(LEP)單克隆抗體 | WB |
| MAA084Hu23 | 瘦素(LEP)單克隆抗體 | WB |
| MAA084Hu24 | 瘦素(LEP)單克隆抗體 | IHC |
| MAA084Hu25 | 瘦素(LEP)單克隆抗體 | IHC |
| HEA084Hu | 瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA084Hu | 瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA084Hu | 瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA084Hu | 瘦素(LEP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA084Hu | 瘦素(LEP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA084Hu01 | 瘦素(LEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women[] |
| PPAR Research | Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment?…[34306045] |
| Br J Nutr | The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study[34348809] |
| Ann Med Surg (Lond) | Maternal and fetal serum leptin levels and their association with maternal and fetal variables and labor: A cross-sectional study[34815864] |
| Biofactors | Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat[34767670] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity[34853313] |
| Int J Mol Sci | The Effects of Separate and Combined Treatment of Male Rats with Type 2 Diabetes with Metformin and Orthosteric and Allosteric Agonists of Luteinizing Hormone?…[Pubmed:35008624] |
| Bull Exp Biol Med | Comparative Study of the Restoring Effect of Metformin, Gonadotropin, and Allosteric Agonist of Luteinizing Hormone Receptor on Spermatogenesis in Male Rats with?…[Pubmed:35175480] |
| Naunyn Schmiedebergs Arch Pharmacol | Intragastric safflower yellow and its main component HSYA improve leptin sensitivity before body weight change in diet-induced obese mice[Pubmed:35201390] |
| npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal[Pubmed:35379849] |
| Ecotoxicol Environ Saf | Monochromatic blue light not green light exposure is associated with continuous light-induced hepatic steatosis in high fat diet fed-mice via oxidative stress[Pubmed:35588616] |
| Biomed Res Int | Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model[Pubmed:35509713] |
| Endocr J | A novel anti-obesity mechanism for liraglutide by improving adipose tissue leptin resistance in high-fat diet-fed obese mice[Pubmed:35705299] |
| Impact of pre-breeding feeding practices on rabbit mammary gland development at mid-pregnancy.[] | |
| High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients[] | |
| Dairy Cattle Physiology Division ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India Corresponding Authors Email ID: menalsh08vets?…[] | |
| Scientific Reports | SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.[] |
