玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Vitronectin (VTN)
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
- 編號(hào)KSB041Ra01
- 物種Rattus norvegicus (Rat,大鼠)相同的名稱,不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Plasma.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍7.81-500ng/mL
- 可適靈敏度3.7ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7798 ¥ 12996¥ 23393¥ 45486¥ 77976
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特異性
試劑盒中的抗體對(duì)玻連蛋白(VTN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB041Ra01 | 玻連蛋白(VTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB041Ra01 | 玻連蛋白(VTN)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB041Ra01 | 玻連蛋白(VTN)多克隆抗體 | WB; IHC; ICC; IP. |
MAB041Ra21 | 玻連蛋白(VTN)單克隆抗體 | WB; IHC; ICC; IP. |
SEB041Ra | 玻連蛋白(VTN)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB041Ra | 玻連蛋白(VTN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB041Ra01 | 玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer[IEEE: 6098563] |
PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance[PubMed: PMC3754973] |
Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE[Pubmed: 23944848] |
Analytical Biochemistry | A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots[Pubmed:29154788] |
Journal of Materials Chemistry B | Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs[Doi: 10.1039/C8TB02283F?] |
JOURNAL OF NEPHROLOGY | Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts[33275196] |