粘蛋白2(MUC2)多克隆抗體
Polyclonal Antibody to Mucin 2 (MUC2)
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal
- 編號PAA705Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPA705Ra01-粘蛋白2(MUC2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 616¥ 1436¥ 2052¥ 5130¥ 20520
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產品包裝(模擬)
產品包裝(模擬)
DAB staining on IHC-P; Sample: Rat Colon Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Colon Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Rabbit Anti-Rat MUC2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Western Blot; Sample: Lane1: Rat Small intestine lysate; Lane2: Mouse Colon lysate; Lane3: Porcine Colon lysate Primary Ab: 0.2μg/ml Rabbit Anti-Rat MUC2 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對MUC2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別MUC2。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA705Ra02 | 粘蛋白2(MUC2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA705Ra01 | 粘蛋白2(MUC2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA705Ra01 | 粘蛋白2(MUC2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA705Ra21 | 粘蛋白2(MUC2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA705Ra | 粘蛋白2(MUC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA705Ra | 粘蛋白2(MUC2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.[PubMed: 20717734] |
| World J Gastroenterol. | Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats.[Pubmed:24627600] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis[PubMed: 26217323] |
| nutrition research and practice | Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway[PubMed: 26244071] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis[PubMed: 26172851] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization[doi:10.1513] |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Bio-protocol | Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA[10.21769:BioProtoc.2879] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease[Pubmed: 30573494] |
| Biomolecules | Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer[Pubmed: 30682816] |
| Cell Stem Cell | PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism[Pubmed: 31564549] |
| DIGESTIVE DISEASES AND SCIENCES | Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats[Pubmed: 32144600] |
| Scientific Reports | Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli[Pubmed: 32601325] |
| Biorxiv | Defective intestinal repair after short-term high fat diet due to loss of efferocytosis[] |
| J Cell Mol Med | Inhibition of cyclooxygenase?\2 enhanced intestinal epithelial homeostasis via suppressing |??\catenin signalling pathway in experimental liver fibrosis[34145945] |
| Cell Tissue Res | Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced?…[34370080] |