Interleukin-6 (IL-6), a pro-inflammatory?cytokine?and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL6 induces C-reactive protein (CRP) expression through the STAT3 pathway in HepG2 cells. To test this effect, HepG2 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without various concentrations of IL6 for 24h and CRP levels in the cell supernatant were determined by ELISA.
CRP levels in the cell supernatant of HepG2 cells increased significantly after stimulated with IL6, the data was shown in Table 1 and Figure 1.