谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白
Active Glutathione S Transferase Pi (GSTp)
GSTP1; GST-P1; DFN7; FAEES3; GST3
- 編號(hào)APB090Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)7.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
Figure. Western Blot
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Glutathione S-transferase P (GSTP1) is a member of Glutathione S-transferases (GSTs) family which play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340nm. The reaction was performed in adding 10μL 200mM glutathione (reduced) and 10μL 100mM CDNB in 980μL 100mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50μL mixed substrates to 50μL recombinant rat GSTP1, mix gentliy, read the absorbance at 340nm for 5min. One unit activity was defined as 1 μmole of CDNB conjugate per minute per 1 mg GSTP1
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB090Ra71 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB090Ra | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB090Ra | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB090Ra01 | 谷胱甘肽S轉(zhuǎn)移酶π1(GSTp)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma[PubMed: PMC3619117] |
| Mol Neurobiol | Human Glutathione S-Transferase Enzyme Gene Polymorphisms and Their Association With Neurocysticercosis[Pubmed:27021019] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering[art00007] |
| Neuroscience?Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state[Pubmed:30019218] |
| Arabian Journal of Chemistry | Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced hepatotoxicity in rats[] |
| International Journal of Nature and Life Sciences | The Role of Glutathione S-Transferases in Pleomorphic Adenomas of the Salivary Glands[] |