胱天蛋白酶3(CASP3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Caspase 3 (CASP3)
CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
- 編號(hào)KSA626Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.056ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)胱天蛋白酶3(CASP3)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA626Hu01 | 胱天蛋白酶3(CASP3)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA626Hu01 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA626Hu02 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA626Hu03 | 胱天蛋白酶3(CASP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA626Hu03 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB |
| PAA626Hu02 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB; IHC |
| PAA626Hu01 | 胱天蛋白酶3(CASP3)多克隆抗體 | WB; IHC |
| LAA626Hu81 | 胱天蛋白酶3(CASP3)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA626Hu71 | 胱天蛋白酶3(CASP3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA626Hu21 | 胱天蛋白酶3(CASP3)單克隆抗體 | WB; IHC |
| MAA626Hu22 | 胱天蛋白酶3(CASP3)單克隆抗體 | WB |
| SEA626Hu | 胱天蛋白酶3(CASP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA626Hu | 胱天蛋白酶3(CASP3)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA626Hu01 | 胱天蛋白酶3(CASP3)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
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| Original Investigation | Neuroprotective Effects of Pregabalin Against Spinal Cord Ischemia-Reperfusion Injury in Rats[JTNEPUB:17959] |
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| Molecular Medicine Reports | Hydrogen sulfide attenuates doxorubicin-induced cardiotoxicity by inhibiting the expression of peroxiredoxin III in H9c2 cells[mmr:13] |
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| Life?Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats[Pubmed: 30205096] |
| AAPS?PharmSciTech | Augmentation of Fluvastatin Cytotoxicity Against Prostate Carcinoma PC3 Cell Line Utilizing Alpha Lipoic–Ellagic Acid Nanostructured Lipid Carrier Formula[Pubmed: 30350252] |
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| Scientific Reports | Mebendazole augments sensitivity to sorafenib by targeting MAPK and BCL-2 signalling in n-nitrosodiethylamine-induced murine hepatocellular carcinoma[Pubmed: 31836811] |
| Pharmaceutics | Preparation and Optimization of In Situ Gel Loaded with Rosuvastatin-Ellagic Acid Nanotransfersomes to Enhance the Anti-Proliferative Activity[Pubmed: 32183144] |
| Photodiagnosis and Photodynamic Therapy | In vitro evaluation of cytotoxic properties of 5-Aminolevulinic acid (5-ALA) on bladder cancer cells[Pubmed: 32165337] |
| Frontiers in Neuroscience | Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus[Pubmed: 32218719] |
| FASEB J | Bovine colostrum induces the differentiation of human primary keratinocytes[Pubmed: 32157742] |
| Marine Drugs | In Situ Gel Loaded with Chitosan-Coated Simvastatin Nanoparticles: Promising Delivery for Effective Anti-Proliferative Activity against Tongue Carcinoma[Pubmed: 32283782] |
| NAUNYN-SCHMIEDEBERGS?ARCHIVES OF?PHARMACOLOGY | Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in rats[Pubmed: 32936352] |
| Pharmaceutics | Chitosan-Based Microparticles Enhance Ellagic Acid's Colon Targeting and Proapoptotic Activity[Pubmed: 32660035] |
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway.[Pubmed: 32603817] |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis[Pubmed: 32565124] |
| International Journal of Molecular Sciences | Optimized Conjugation of Fluvastatin to HIV-1 TAT Displays Enhanced Pro-Apoptotic Activity in HepG2 Cells[Pubmed: 32531976] |
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| Iranian Journal of Basic Medical Sciences | Chromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemia[Pubmed: 32963739] |
| International Journal of Research in Pharmaceutical | In situ gel loaded with curcumin-based fluvastatin nanostructured carrier effective against squamous tongue carcinoma[] |
| SAGE Journals | Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic?…[] |
| Bangladesh Journal of Pharmacology | Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat[] |
| International Journal of Pharmacology | Ellagic Acid Loaded TPGS Micelles for Enhanced Anticancer Activities in Ovarian Cancer[] |
| Strawberry (Fragaria x ananassa) leaf extract reduces brain damage in diabetic male rats[] | |
| Indian Journal of Animal Research | Accacia senegel extract ameliorates Lead Acetate-mediated Hepatotoxicity in rats.[] |
| RESEARCH SQUARE | Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs[] |
| Egyptian Journal of Basic and Applied Sciences | Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats[] |
| ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES[] | |
| Norwegian Journal of development of the International Science | CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER [] |
| J Biochem Mol Toxicol | Concanavalin A induces apoptosis in a dose?\dependent manner by modulating thiol/disulfide homeostasis in C6 glioblastoma cells[33604990] |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis[33575317] |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats[33750916] |
| TOXICOLOGY IN VITRO | Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells[33675893] |
| Biomed Pharmacother | Captopril downregulates expression of Bax/cytochrome C/caspase-3 apoptotic pathway, reduces inflammation, and oxidative stress in cisplatin-induced acute??-[33945910] |
| Reprod Sci | Reproductive Effects of Nicotinamide on Testicular Function and Structure in Old Male Rats: Oxidative, Apoptotic, Hormonal, and Morphological Analyses[34101148] |
| BMC Complement Med Ther | Effect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitis[34103031] |
| Environ Sci Pollut Res Int | Comparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological??-[34097215] |
| Piceatannol Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Modulation of Nrf2/HO-1/NF|êB Axis. Front. Pharmacol. 11: 614897. doi??-[33519479] | |
| Biomed Pharmacother | Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats[34426252] |
| Chem Biol Interact | Protective role of naringin loaded solid nanoparticles against aflatoxin B1 induced hepatocellular carcinoma[34717916] |
| Biochim Biophys Acta Gen Subj | Paraoxonase 2 protects against the CML mediated mitochondrial dysfunction through modulating JNK pathway in human retinal cells[34710487] |
| AAPS PharmSciTech | Development of an Icariin-Loaded Bilosome-Melittin Formulation with Improved Anticancer Activity against Cancerous Pancreatic Cells[34668089] |
| Analytical Cellular Pathology | The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia[34336554] |
| Ecotoxicol Environ Saf | Evaluation of cytogenotoxic potential and embryotoxicity of KRS-Cauvery River water in zebrafish (Danio rerio)[Pubmed:35183813] |
| Environ Sci Pollut Res Int | Chrysin attenuates estradiol-induced endometrial hyperplasia in rats via enhancing PPARα activity[Pubmed:35301625] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats[Pubmed:35421180] |
| Journal of Ethnopharmacology | Ethnopharmacological impact of Melaleuca rugulosa (Link) Craven leaves extract on liver inflammation[Pubmed:35337921] |
| Eur J Pharmacol | Harmine prevents 3-nitropropionic acid-induced neurotoxicity in rats via enhancing NRF2-mediated signaling: Involvement of p21 and AMPK[Pubmed:35623405] |
| Gels | QbD Supported Optimization of the Alginate-Chitosan Nanoparticles of Simvastatin in Enhancing the Anti-Proliferative Activity against Tongue Carcinoma[Pubmed:35200484] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?…[Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?…[Pubmed:35204140] |
| Value Health Sci | Oksidatif, Apoptotik ve ?nflamatuar Sinyal Yolaklar? üzerinden C6 Glioblastoma Hücrelerindeki ML351'in Antiproliferatif Etkileri[] |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?…[] | |
| Biointerface Research in Applied Chemistry | Promising Therapeutic Efficacy of Trigonella-foenum graecum and Bone Marrow-Derived Mesenchymal Stem Cells on Skeletal Muscle Atrophy in Experimental?…[] |
| Arab Journal of Nuclear Sciences and Applications | Potential activity of silver/hydroxyapatite nano composite against damaged liver consequence to irradiation[] |